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X-ray diffraction
2Å resolution

E.COLI METHIONINE AMINOPEPTIDASE TRANSITION-STATE INHIBITOR COMPLEX

Released:
DOI: 10.2210/pdb3mat/pdb
PDB entry 3mat coloured by chain, front view.
PDB entry 3mat coloured by chain, side view.
PDB entry 3mat coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis.
Lowther WT, Orville AM, Madden DT, Lim S, Rich DH, Matthews BW
Biochemistry 38 7678-88 (1999)
PMID: 10387007

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142398 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methionine aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE18 (Residues: 1-264; Coverage: 100%)
Gene names: JW0163, b0168, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily
bestatin-based inhibitor (3R)-amino-(2S)-hydroxyheptanoyl-l-Ala-l-Leu-l-Val-l-Phe-OMe Chain: I
Molecule details ›
Chain: I
Length: 5 amino acids
Theoretical weight: 606 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand NA 1 x NA
2 modified residues:
Ligand AHH 1 x AHH
Ligand 0A9 1 x 0A9

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 39.297Å b: 65.155Å c: 51.371Å
α: 90° β: 106.12° γ: 90°
R-values:
R R work R free
0.156 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Protein-reactive natural products.
Drahl et al. (2005)
5 more

9 mentions without citation

Mutations in pepQ Confer Low-Level Resistance to Bedaquiline and Clofazimine in Mycobacterium tuberculosis.
Almeida et al. (2016)
8 more