› 3mau

X-ray diffraction
2.9Å resolution

Crystal structure of StSPL in complex with phosphoethanolamine

Released:
DOI: 10.2210/pdb3mau/pdb
PDB entry 3mau coloured by chain, front view.
PDB entry 3mau coloured by chain, side view.
PDB entry 3mau coloured by chain, top view.
Source organism: Symbiobacterium thermophilum
Primary publication:
Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.
Bourquin F, Riezman H, Capitani G, Grütter MG
Structure 18 1054-65 (2010)
PMID: 20696404

Function and Biology Details

Reaction catalysed: 
Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sphingosine-1-phosphate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 514 amino acids
Theoretical weight: 55.57 KDa
Source organism: Symbiobacterium thermophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q67PY4 (Residues: 2-507; Coverage: 100%)
Gene name: STH1274
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLR 1 x PLR
2 bound ligands:
Ligand PO4 1 x PO4
Ligand EXT 3 x EXT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 64.36Å b: 243.45Å c: 280.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.252
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Targeting the sphingosine-1-phosphate axis in cancer, inflammation and beyond.
Kunkel et al. (2013)
19 more

4 mentions without citation

Sphingosine-1-phosphate metabolism: A structural perspective.
Pulkoski-Gross et al. (2015)
3 more