› 3mb8

X-ray diffraction
1.9Å resolution

Crystal structure of purine nucleoside phosphorylase from toxoplasma gondii in complex with immucillin-H

Released:
DOI: 10.2210/pdb3mb8/pdb
PDB entry 3mb8 coloured by chain, front view.
PDB entry 3mb8 coloured by chain, side view.
PDB entry 3mb8 coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Inhibition and structure of Toxoplasma gondii purine nucleoside phosphorylase.
Donaldson TM, Cassera MB, Ho MC, Zhan C, Merino EF, Evans GB, Tyler PC, Almo SC, Schramm VL, Kim K
Eukaryot Cell 13 572-9 (2014)
PMID: 24585883

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside phosphorylase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.58 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2HXR2 (Residues: 1-247; Coverage: 100%)
Gene name: PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand IMH 2 x IMH
Ligand PO4 2 x PO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6
Unit cell:
a: 159.622Å b: 159.622Å c: 53.606Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.221 0.243
Expression system: Escherichia coli

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Citations

1 review citation

Nucleoside Hydrolase NH 36: A Vital Enzyme for the Leishmania Genus in the Development of T-Cell Epitope Cross-Protective Vaccines.
Palatnik-de-Sousa CB. (2019)
9 other articles

8 mentions without citation

Drugs in development for toxoplasmosis: advances, challenges, and current status.
Alday et al. (2017)
7 more