› 3mbe

X-ray diffraction
2.89Å resolution

TCR 21.30 in complex with MHC class II I-Ag7HEL(11-27)

Released:
DOI: 10.2210/pdb3mbe/pdb
PDB entry 3mbe coloured by chain, front view.
PDB entry 3mbe coloured by chain, side view.
PDB entry 3mbe coloured by chain, top view.
Source organisms:
Primary publication:
The diabetogenic mouse MHC class II molecule I-Ag7 is endowed with a switch that modulates TCR affinity.
Yoshida K, Corper AL, Herro R, Jabri B, Wilson IA, Teyton L
J Clin Invest 120 1578-90 (2010)
PMID: 20407212

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-132848 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
H-2 class II histocompatibility antigen, A-D alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 190 amino acids
Theoretical weight: 21.62 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P04228 (Residues: 24-205; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chains: B, F
Molecule details ›
Chains: B, F
Length: 201 amino acids
Theoretical weight: 23.34 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q31135 (Residues: 28-214; Coverage: 79%)
Gene name: H2-Ab1
Sequence domains:
Structure domains:
Lysozyme C Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 18 amino acids
Theoretical weight: 2.01 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00698 (Residues: 28-45; Coverage: 14%)
Gene name: LYZ
T-cell receptor alpha chain constant Chains: C, G
Molecule details ›
Chains: C, G
Length: 229 amino acids
Theoretical weight: 25.39 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01849 (Residues: 1-115; Coverage: 85%)
Gene name: Trac
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T-cell receptor beta-1 chain C region Chains: D, H
Molecule details ›
Chains: D, H
Length: 259 amino acids
Theoretical weight: 28.95 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01852 (Residues: 1-136; Coverage: 79%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 67.468Å b: 99.391Å c: 404.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.253 0.281
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

7 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
6 more

14 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
13 more