› 3mbk

X-ray diffraction
1.35Å resolution

The 1.35 A Structure of the Phosphatase Domain of the Suppressor of T Cell Receptor Signalling Protein in Complex with Sulphate

Released:
DOI: 10.2210/pdb3mbk/pdb
PDB entry 3mbk coloured by chain, front view.
PDB entry 3mbk coloured by chain, side view.
PDB entry 3mbk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The 1.35 A resolution structure of the phosphatase domain of the suppressor of T-cell receptor signaling protein in complex with sulfate.
Jakoncic J, Sondgeroth B, Carpino N, Nassar N
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 643-7 (2010)
PMID: 20516590

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-associated and SH3 domain-containing protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8BGG7 (Residues: 373-636; Coverage: 41%)
Gene names: Sts1, Ubash3b
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 62.966Å b: 80.049Å c: 105.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structural and Functional Characterization of the Histidine Phosphatase Domains of Human Sts-1 and Sts-2.
Zhou et al. (2017)

1 mention without citation

The 1.35 A resolution structure of the phosphatase domain of the suppressor of T-cell receptor signaling protein in complex with sulfate.
Jakoncic et al. (2010)