› 3mco

X-ray diffraction
2.3Å resolution

Crystal Structure of the 6-hyroxymethyl-7,8-dihydropterin pyrophosphokinase dihydropteroate synthase bifunctional enzyme from Francisella tularensis

Released:
DOI: 10.2210/pdb3mco/pdb
PDB entry 3mco coloured by chain, front view.
PDB entry 3mco coloured by chain, side view.
PDB entry 3mco coloured by chain, top view.
Source organism: Francisella tularensis subsp. holarctica LVS
Primary publication:
Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase•dihydropteroate synthase bifunctional enzyme from Francisella tularensis.
Pemble CW, Mehta PK, Mehra S, Li Z, Nourse A, Lee RE, White SW
PLoS One 5 e14165 (2010)
PMID: 21152407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 50.59 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PH2 4 x PH2
Ligand APC 2 x APC
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 42.521Å b: 58.472Å c: 109.327Å
α: 82° β: 81° γ: 68.08°
R-values:
R R work R free
0.243 0.241 0.294
Expression system: Escherichia coli

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Citations

3 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
2 more

3 mentions without citation

Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase•dihydropteroate synthase bifunctional enzyme from Francisella tularensis.
Pemble et al. (2010)
2 more