› 3mdu

X-ray diffraction
1.4Å resolution

The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-Guanidino-L-Glutamate

Released:
DOI: 10.2210/pdb3mdu/pdb
PDB entry 3mdu coloured by chain, front view.
PDB entry 3mdu coloured by chain, side view.
PDB entry 3mdu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa.
Fedorov AA, Martí-Arbona R, Nemmara VV, Hitchcock D, Fedorov EV, Almo SC, Raushel FM
Biochemistry 54 890-7 (2015)
PMID: 25559274

Function and Biology Details

Reaction catalysed: 
N-formimidoyl-L-glutamate + H(2)O = N-formyl-L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-190952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formimidoylglutamate deiminase Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 49.4 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HU77 (Residues: 1-453; Coverage: 100%)
Gene names: PA5106, hutF
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand NGQ 1 x NGQ
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I4122
Unit cell:
a: 133.208Å b: 133.208Å c: 124.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.189
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structure of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa.
Fedorov et al. (2015)