› 3meb

X-ray diffraction
1.9Å resolution

Structure of cytoplasmic aspartate aminotransferase from giardia lamblia

Released:
DOI: 10.2210/pdb3meb/pdb
PDB entry 3meb coloured by chain, front view.
PDB entry 3meb coloured by chain, side view.
PDB entry 3meb coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Wall A, Clifton MC, Lukacs CM, Staker BL, Van Voorhis W, Myler P, Lorimer DD, Edwards TE
Acta Crystallogr F Struct Biol Commun 71 566-71 (2015)
PMID: 25945710

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 49.97 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli
UniProt:
  • Canonical: A8B1V5 (Residues: 1-427; Coverage: 100%)
Gene names: GL50803_0091056, GL50803_91056
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 14 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 58.58Å b: 101.15Å c: 81.53Å
α: 90° β: 90.62° γ: 90°
R-values:
R R work R free
0.176 0.174 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids.
Marchese et al. (2018)
1 more

2 mentions without citation

Structural Insights into a Novel Class of Aspartate Aminotransferase from Corynebacterium glutamicum.
Son et al. (2016)
1 more