› 3mex

X-ray diffraction
2.1Å resolution

Crystal structure of MexR in oxidized state

Released:
DOI: 10.2210/pdb3mex/pdb
PDB entry 3mex coloured by chain, front view.
PDB entry 3mex coloured by chain, side view.
PDB entry 3mex coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR.
Chen H, Yi C, Zhang J, Zhang W, Ge Z, Yang CG, He C
EMBO Rep 11 685-90 (2010)
PMID: 20616806

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.4 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52003 (Residues: 1-142; Coverage: 97%)
Gene names: PA0424, mexR
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 39.554Å b: 72.183Å c: 98.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
9 more

2 mentions without citation

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more