› 3mf8

X-ray diffraction
2.01Å resolution

Crystal Structure of Native cis-CaaD

Released:
DOI: 10.2210/pdb3mf8/pdb
Model geometry
Fit model/data
PDB entry 3mf8 coloured by chain, front view.
PDB entry 3mf8 coloured by chain, side view.
PDB entry 3mf8 coloured by chain, top view.
Source organism: coryneform bacterium
Primary publication:
Crystal structures of native and inactivated cis-3-chloroacrylic acid dehalogenase: Implications for the catalytic and inactivation mechanisms.
Guo Y, Serrano H, Johnson WH, Ernst S, Hackert ML, Whitman CP
Bioorg Chem 39 1-9 (2011)
PMID: 21074239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tautomerase cis-CaaD-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.64 KDa
Source organism: coryneform bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6VPE5 (Residues: 2-150; Coverage: 99%)
Gene name: cis-caaD
Sequence domains: Putative oxalocrotonate tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P63
Unit cell:
a: 58.987Å b: 58.987Å c: 58.297Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.236
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Reactions of Cg10062, a cis-3-Chloroacrylic Acid Dehalogenase Homologue, with Acetylene and Allene Substrates: Evidence for a Hydration-Dependent Decarboxylation.
Huddleston et al. (2015)

3 mentions without citation

Kinetic and structural characterization of a cis-3-Chloroacrylic acid dehalogenase homologue in Pseudomonas sp. UW4: A potential step between subgroups in the tautomerase superfamily.
LeVieux et al. (2017)
2 more