› 3mfe

X-ray diffraction
2.6Å resolution

Crystal Structure of Mycobacterium Tuberculosis Proteasome open-gate mutant with H0 movement

Released:
DOI: 10.2210/pdb3mfe/pdb
Model geometry
Fit model/data
PDB entry 3mfe coloured by chain, front view.
PDB entry 3mfe coloured by chain, side view.
PDB entry 3mfe coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasome.
Li D, Li H, Wang T, Pan H, Lin G, Li H
EMBO J 29 2037-47 (2010)
PMID: 20461058

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161584 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit beta Chains: 2, C, E, H, J, L, N, P, R, T, X, Z
Molecule details ›
Chains: 2, C, E, H, J, L, N, P, R, T, X, Z
Length: 240 amino acids
Theoretical weight: 25.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: G, V
Molecule details ›
Chains: G, V
Length: 240 amino acids
Theoretical weight: 25.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 118.968Å b: 207.554Å c: 142.275Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.224 0.224 0.257
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Proteasome activators.
Stadtmueller et al. (2011)
12 more

2 mentions without citation

The pup-proteasome system of Mycobacterium tuberculosis.
Samanovic et al. (2013)
1 more