› 3mgo

X-ray diffraction
2.3Å resolution

Crystal structure of a H5-specific CTL epitope derived from H5N1 influenza virus in complex with HLA-A*0201

Released:
DOI: 10.2210/pdb3mgo/pdb
PDB entry 3mgo coloured by chain, front view.
PDB entry 3mgo coloured by chain, side view.
PDB entry 3mgo coloured by chain, top view.
Source organisms:
Primary publication:
Identification and structural definition of H5-specific CTL epitopes restricted by HLA-A*0201 derived from the H5N1 subtype of influenza A viruses.
Sun Y, Liu J, Yang M, Gao F, Zhou J, Kitamura Y, Gao B, Tien P, Shu Y, Iwamoto A, Chen Z, Gao GF
J Gen Virol 91 919-30 (2010)
PMID: 19955560

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137810 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Hemagglutinin HA1 chain Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 10 amino acids
Theoretical weight: 1.27 KDa
Source organism: H5N1 subtype
Expression system: Not provided
UniProt:
  • Canonical: Q2F4V2 (Residues: 205-214; Coverage: 2%)
Gene name: HA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 63.211Å b: 79.322Å c: 87.146Å
α: 90° β: 90° γ: 90.02°
R-values:
R R work R free
0.204 0.202 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

The antigenic architecture of the hemagglutinin of influenza H5N1 viruses.
Velkov et al. (2013)
4 more

8 mentions without citation

Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope.
Song et al. (2017)
7 more