› 3mgv

X-ray diffraction
2.29Å resolution

Cre recombinase-DNA transition state

Released:
DOI: 10.2210/pdb3mgv/pdb
PDB entry 3mgv coloured by chain, front view.
PDB entry 3mgv coloured by chain, side view.
PDB entry 3mgv coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Requirements for catalysis in the Cre recombinase active site.
Gibb B, Gupta K, Ghosh K, Sharp R, Chen J, Van Duyne GD
Nucleic Acids Res 38 5817-32 (2010)
PMID: 20462863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116569 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombinase cre Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 343 amino acids
Theoretical weight: 38.6 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli
UniProt:
  • Canonical: P06956 (Residues: 1-343; Coverage: 100%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
DNA (5'-D(*TP*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*G)-3') Chains: E, G, I, L
Molecule details ›
Chains: E, G, I, L
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Punavirus P1
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*AP*TP*GP*CP*TP*AP*TP*AP*CP*GP*AP*AP*GP*TP*TP*AP*T)-3') Chains: F, H, K, M
Molecule details ›
Chains: F, H, K, M
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand VO4 2 x VO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3221
Unit cell:
a: 136.046Å b: 136.046Å c: 218.431Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Mechanisms of DNA Transposition.
Hickman et al. (2015)
5 more

4 mentions without citation

Targeted gene disruption to cure HIV.
Stone et al. (2013)
3 more