› 3mii

X-ray diffraction
2.4Å resolution

Crystal structure of Y0R391Cp/HSP33 from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3mii/pdb
PDB entry 3mii coloured by chain, front view.
PDB entry 3mii coloured by chain, side view.
PDB entry 3mii coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of Hsp33/YOR391Cp from the yeast Saccharomyces cerevisiae.
Guo PC, Zhou YY, Ma XX, Li WF
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 1557-61 (2010)
PMID: 21139195

Function and Biology Details

Reaction catalysed: 
(R)-lactate = methylglyoxal + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable glutathione-independent glyoxalase HSP33 Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.99 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08914 (Residues: 1-237; Coverage: 100%)
Gene names: HSP33, YOR391C
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand PGE 2 x PGE
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 96.42Å b: 96.42Å c: 132.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe.
Zhao et al. (2014)
1 more

3 mentions without citation

A glutathione-independent glyoxalase of the DJ-1 superfamily plays an important role in managing metabolically generated methylglyoxal in Candida albicans.
Hasim et al. (2014)
2 more