› 3mip

X-ray diffraction
2.4Å resolution

I-MsoI re-designed for altered DNA cleavage specificity (-8GCG)

Released:
DOI: 10.2210/pdb3mip/pdb
PDB entry 3mip coloured by chain, front view.
PDB entry 3mip coloured by chain, side view.
PDB entry 3mip coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Ashworth J, Taylor GK, Havranek JJ, Quadri SA, Stoddard BL, Baker D
Nucleic Acids Res 38 5601-8 (2010)
PMID: 20435674

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-116571 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mso-8G Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 18.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Homing endonucleases
DNA (5'-D(*GP*CP*AP*GP*GP*CP*GP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*CP*GP*CP*TP*CP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.41 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*GP*AP*GP*CP*GP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*CP*GP*CP*CP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.33 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 42.539Å b: 42.611Å c: 71.524Å
α: 96.45° β: 107.04° γ: 108.56°
R-values:
R R work R free
0.232 0.226 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.
Stoddard BL. (2011)
21 more

1 mention without citation

Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Ashworth et al. (2010)