› 3miw

X-ray diffraction
2.5Å resolution

Crystal Structure of Rotavirus NSP4

Released:
DOI: 10.2210/pdb3miw/pdb
PDB entry 3miw coloured by chain, front view.
PDB entry 3miw coloured by chain, side view.
PDB entry 3miw coloured by chain, top view.
Source organism: Human rotavirus G4 strain St. Thomas 3
Primary publication:
A new pentameric structure of rotavirus NSP4 revealed by molecular replacement.
Chacko AR, Jeyakanthan J, Ueno G, Sekar K, Rao CD, Dodson EJ, Suguna K, Read RJ
Acta Crystallogr D Biol Crystallogr 68 57-61 (2012)
PMID: 22194333

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural glycoprotein 4 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 53 amino acids
Theoretical weight: 6.51 KDa
Source organism: Human rotavirus G4 strain St. Thomas 3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82035 (Residues: 95-146; Coverage: 30%)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand EDO 27 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P42
Unit cell:
a: 63.463Å b: 63.463Å c: 123.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.261 0.297
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Viroporins: structure, function and potential as antiviral targets.
Scott et al. (2015)
2 other articles

6 mentions without citation

Rotavirus non-structural proteins: structure and function.
Hu et al. (2012)
5 more