› 3mlh

X-ray diffraction
2.09Å resolution

Crystal structure of the 2009 H1N1 influenza virus hemagglutinin receptor-binding domain

Released:
DOI: 10.2210/pdb3mlh/pdb
PDB entry 3mlh coloured by chain, front view.
PDB entry 3mlh coloured by chain, side view.
PDB entry 3mlh coloured by chain, top view.
Source organism: Influenza A virus (A/Mexico/4603/2009(H1N1))
Primary publication:
The receptor-binding domain of influenza virus hemagglutinin produced in Escherichia coli folds into its native, immunogenic structure.
DuBois RM, Aguilar-Yañez JM, Mendoza-Ochoa GI, Oropeza-Almazán Y, Schultz-Cherry S, Alvarez MM, White SW, Russell CJ
J Virol 85 865-72 (2011)
PMID: 21068239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemagglutinin Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.41 KDa
Source organism: Influenza A virus (A/Mexico/4603/2009(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: C4RSQ3 (Residues: 63-286; Coverage: 40%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 39.857Å b: 74.065Å c: 75.18Å
α: 90° β: 94.73° γ: 90°
R-values:
R R work R free
0.178 0.177 0.206
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Advances in the development of influenza virus vaccines.
Krammer et al. (2015)
4 more

7 mentions without citation

Hemagglutinin Stability and Its Impact on Influenza A Virus Infectivity, Pathogenicity, and Transmissibility in Avians, Mice, Swine, Seals, Ferrets, and Humans.
Russell CJ. (2021)
6 more