3mlp

X-ray diffraction
2.8Å resolution

Early B-cell Factor 1 (Ebf1) bound to DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-116580 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription factor COE1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 402 amino acids
Theoretical weight: 45.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07802 (Residues: 24-421; Coverage: 66%)
Gene names: Coe1, Ebf, Ebf1
Sequence domains:
Structure domains: Immunoglobulins
DNA (5'-D(*CP*TP*TP*TP*AP*TP*TP*CP*CP*CP*AP*TP*GP*GP*GP*AP*AP*TP*AP*AP*AP*G)-3') Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P1
Unit cell:
a: 70.49Å b: 78.69Å c: 103.57Å
α: 88.99° β: 90° γ: 77.81°
R-values:
R R work R free
0.207 0.204 0.264
Expression systems:
  • Escherichia coli
  • Not provided