› 3mme

X-ray diffraction
3.97Å resolution

Structure and functional dissection of PG16, an antibody with broad and potent neutralization of HIV-1

Released:
DOI: 10.2210/pdb3mme/pdb
PDB entry 3mme coloured by chain, front view.
PDB entry 3mme coloured by chain, side view.
PDB entry 3mme coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of PG16 and chimeric dissection with somatically related PG9: structure-function analysis of two quaternary-specific antibodies that effectively neutralize HIV-1.
Pancera M, McLellan JS, Wu X, Zhu J, Changela A, Schmidt SD, Yang Y, Zhou T, Phogat S, Mascola JR, Kwong PD
J Virol 84 8098-110 (2010)
PMID: 20538861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213325 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
PG16 HEAVY CHAIN FAB Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 238 amino acids
Theoretical weight: 25.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PG16 LIGHT CHAIN FAB Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 216 amino acids
Theoretical weight: 22.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C222
Unit cell:
a: 191.961Å b: 230.804Å c: 82.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.256 0.317
Expression system: Homo sapiens

Quick links

Citations

28 review citations

HIV-1 neutralizing antibodies: understanding nature's pathways.
Mascola et al. (2013)
27 more

1 mention without citation

Crystal structure of PG16 and chimeric dissection with somatically related PG9: structure-function analysis of two quaternary-specific antibodies that effectively neutralize HIV-1.
Pancera et al. (2010)