› 3mmn

X-ray diffraction
2.2Å resolution

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+

Released:
DOI: 10.2210/pdb3mmn/pdb
PDB entry 3mmn coloured by chain, front view.
PDB entry 3mmn coloured by chain, side view.
PDB entry 3mmn coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
Pekárová B, Klumpler T, Třísková O, Horák J, Jansen S, Dopitová R, Borkovcová P, Papoušková V, Nejedlá E, Sklenář V, Marek J, Zídek L, Hejátko J, Janda L
Plant J 67 827-39 (2011)
PMID: 21569135

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine kinase CKI1 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.23 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22267 (Residues: 944-1122; Coverage: 16%)
Gene names: At2g47430, CKI1, T30B22.27
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 54.142Å b: 100.915Å c: 80.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Organization and mode of action of two component system signaling circuits from the various kingdoms of life.
Alvarez et al. (2016)
4 more

4 mentions without citation

Modeling of Protein⁻Protein Interactions in Cytokinin Signal Transduction.
Arkhipov et al. (2019)
3 more