› 3mn5

X-ray diffraction
1.5Å resolution

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Released:
DOI: 10.2210/pdb3mn5/pdb
PDB entry 3mn5 coloured by chain, front view.
PDB entry 3mn5 coloured by chain, side view.
PDB entry 3mn5 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.
Ducka AM, Joel P, Popowicz GM, Trybus KM, Schleicher M, Noegel AA, Huber R, Holak TA, Sitar T
Proc Natl Acad Sci U S A 107 11757-62 (2010)
PMID: 20538977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 40.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 95%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Protein spire Chain: S
Molecule details ›
Chain: S
Length: 38 amino acids
Theoretical weight: 4.31 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U1K1 (Residues: 448-485; Coverage: 4%)
Gene names: CG10076, p150-Spir, spir

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
Ligand LAB 1 x LAB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 52.96Å b: 71.81Å c: 100.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.216
Expression system: Escherichia coli

Quick links

Citations

4 review citations

New mechanisms and functions of actin nucleation.
Firat-Karalar et al. (2011)
3 more

7 mentions without citation

Actin monomers activate inverted formin 2 by competing with its autoinhibitory interaction.
Ramabhadran et al. (2013)
6 more