› 3mok

X-ray diffraction
1.55Å resolution

Structure of Apo HasAp from Pseudomonas aeruginosa to 1.55A Resolution

Released:
DOI: 10.2210/pdb3mok/pdb
PDB entry 3mok coloured by chain, front view.
PDB entry 3mok coloured by chain, side view.
PDB entry 3mok coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural, NMR spectroscopic, and computational investigation of hemin loading in the hemophore HasAp from Pseudomonas aeruginosa.
Jepkorir G, Rodríguez JC, Rui H, Im W, Lovell S, Battaile KP, Alontaga AY, Yukl ET, Moënne-Loccoz P, Rivera M
J Am Chem Soc 132 9857-72 (2010)
PMID: 20572666

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasAp Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 18.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XD33 (Residues: 1-184; Coverage: 90%)
Gene names: PA3407, hasAp
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P21
Unit cell:
a: 32.984Å b: 65.247Å c: 38.343Å
α: 90° β: 110.48° γ: 90°
R-values:
R R work R free
0.154 0.152 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Heme Uptake and Utilization by Gram-Negative Bacterial Pathogens.
Richard et al. (2019)
3 more

10 mentions without citation

Structural, NMR spectroscopic, and computational investigation of hemin loading in the hemophore HasAp from Pseudomonas aeruginosa.
Jepkorir et al. (2010)
9 more