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X-ray diffraction
1.2Å resolution

Structure of dimeric holo HasAp H32A Mutant from Pseudomonas aeruginosa to 1.20A Resolution

Released:
DOI: 10.2210/pdb3mol/pdb
PDB entry 3mol coloured by chain, front view.
PDB entry 3mol coloured by chain, side view.
PDB entry 3mol coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural, NMR spectroscopic, and computational investigation of hemin loading in the hemophore HasAp from Pseudomonas aeruginosa.
Jepkorir G, Rodríguez JC, Rui H, Im W, Lovell S, Battaile KP, Alontaga AY, Yukl ET, Moënne-Loccoz P, Rivera M
J Am Chem Soc 132 9857-72 (2010)
PMID: 20572666

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasAp Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 18.83 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XD33 (Residues: 1-184; Coverage: 90%)
Gene names: PA3407, hasAp
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Unit cell:
a: 71.74Å b: 52.7Å c: 85.714Å
α: 90° β: 91.48° γ: 90°
R-values:
R R work R free
0.178 0.177 0.197
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Heme Uptake and Utilization by Gram-Negative Bacterial Pathogens.
Richard et al. (2019)
3 more

8 mentions without citation

Crystal structure of the mobile metallo-β-lactamase AIM-1 from Pseudomonas aeruginosa: insights into antibiotic binding and the role of Gln157.
Leiros et al. (2012)
7 more