› 3mqt

X-ray diffraction
2.1Å resolution

Crystal structure of a mandelate racemase/muconate lactonizing enzyme from Shewanella pealeana

Released:
DOI: 10.2210/pdb3mqt/pdb
PDB entry 3mqt coloured by chain, front view.
PDB entry 3mqt coloured by chain, side view.
PDB entry 3mqt coloured by chain, top view.
Source organism: Shewanella pealeana ATCC 700345
Entry authors: Agarwal R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 394 amino acids
Theoretical weight: 44.49 KDa
Source organism: Shewanella pealeana ATCC 700345
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8H7M5 (Residues: 2-384; Coverage: 100%)
Gene name: Spea_3246
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 12 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 111.164Å b: 146.142Å c: 158.71Å
α: 98.47° β: 96.8° γ: 105.04°
R-values:
R R work R free
0.186 0.185 0.213
Expression system: Escherichia coli BL21(DE3)

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