› 3msu

X-ray diffraction
1.84Å resolution

Crystal Structure of Citrate Synthase from Francisella tularensis

Released:
DOI: 10.2210/pdb3msu/pdb
PDB entry 3msu coloured by chain, front view.
PDB entry 3msu coloured by chain, side view.
PDB entry 3msu coloured by chain, top view.
Source organism: Francisella tularensis
Entry authors: Maltseva N, Kim Y, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.73 KDa
Source organism: Francisella tularensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NIJ6 (Residues: 1-424; Coverage: 100%)
Gene names: FTT_0071c, gltA
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand OAA 2 x OAA
Ligand ZN 2 x ZN
Ligand CL 1 x CL
Ligand SO4 3 x SO4
Ligand ACY 1 x ACY
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 75.401Å b: 76.377Å c: 154.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.2
Expression system: Escherichia coli

Quick links