Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Papain-like cysteine protease (preferred)
PDBe Complex ID:
PDB-CPX-109770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Papain-like cysteine protease
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.09 KDa
Source organism: Porcine reproductive and respiratory syndrome virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 203 amino acids
Theoretical weight: 23.09 KDa
Source organism: Porcine reproductive and respiratory syndrome virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
B2ZAB4 (Residues: 181-383; Coverage: 8%)
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-5A, PHOTON FACTORY BEAMLINE BL-17A
Spacegroup:
P41212
Expression system: Escherichia coli BL21(DE3)
