› 3mug

X-ray diffraction
2.49Å resolution

Crystal structure of human Fab PG16, a broadly reactive and potent HIV-1 neutralizing antibody

Released:
DOI: 10.2210/pdb3mug/pdb
PDB entry 3mug coloured by chain, front view.
PDB entry 3mug coloured by chain, side view.
PDB entry 3mug coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1.
Pejchal R, Walker LM, Stanfield RL, Phogat SK, Koff WC, Poignard P, Burton DR, Wilson IA
Proc Natl Acad Sci U S A 107 11483-8 (2010)
PMID: 20534513

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213327 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody PG16 Light Chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 216 amino acids
Theoretical weight: 22.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody PG16 Heavy Chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 241 amino acids
Theoretical weight: 26.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
2 modified residues:
Ligand PCA 12 x PCA
Ligand TYS 6 x TYS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 128.77Å b: 66.25Å c: 198.7Å
α: 90° β: 98° γ: 90°
R-values:
R R work R free
0.209 0.207 0.248
Expression system: Homo sapiens

Quick links

Citations

38 review citations

Human antibodies that neutralize HIV-1: identification, structures, and B cell ontogenies.
Kwong et al. (2012)
37 more

4 mentions without citation

Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1.
Pejchal et al. (2010)
3 more