› 3mv2

X-ray diffraction
2.9Å resolution

Crystal Structure of a-COP in Complex with e-COP

Released:
DOI: 10.2210/pdb3mv2/pdb
PDB entry 3mv2 coloured by chain, front view.
PDB entry 3mv2 coloured by chain, side view.
PDB entry 3mv2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat.
Hsia KC, Hoelz A
Proc Natl Acad Sci U S A 107 11271-6 (2010)
PMID: 20534429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154131 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coatomer subunit alpha Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 325 amino acids
Theoretical weight: 36.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53622 (Residues: 900-1201; Coverage: 25%)
Gene names: COP1, D1578, RET1, SEC33, SOO1, YDL145C
Sequence domains: Coatomer (COPI) alpha subunit C-terminus
Coatomer subunit epsilon Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 310 amino acids
Theoretical weight: 35.47 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40509 (Residues: 1-296; Coverage: 100%)
Gene names: SEC28, YIL076W
Sequence domains: Coatomer epsilon subunit
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 329.127Å b: 74.382Å c: 97.247Å
α: 90° β: 102.27° γ: 90°
R-values:
R R work R free
0.246 0.246 0.297
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The structure of the nuclear pore complex.
Hoelz et al. (2011)
10 more

4 mentions without citation

Structures and mechanisms of vesicle coat components and multisubunit tethering complexes.
Jackson et al. (2012)
3 more