› 3mvg

X-ray diffraction
1.25Å resolution

Native structure of IRIP, a type I ribosome inactivating protein from Iris hollandica var. at 1.25 A

Released:
DOI: 10.2210/pdb3mvg/pdb
PDB entry 3mvg coloured by chain, front view.
PDB entry 3mvg coloured by chain, side view.
PDB entry 3mvg coloured by chain, top view.
Source organism: Iris x hollandica
Entry authors: Meyer A, Weber W, Singh TP, Betzel C

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA N-glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.06 KDa
Source organism: Iris x hollandica
UniProt:
  • Canonical: O04358 (Residues: 24-298; Coverage: 100%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 49.879Å b: 84.809Å c: 69.08Å
α: 90° β: 97.67° γ: 90°
R-values:
R R work R free
0.169 0.168 0.185

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