› 3mw8

X-ray diffraction
1.65Å resolution

Crystal structure of an UROPORPHYRINOGEN-III SYNTHASE (Sama_3255) from SHEWANELLA AMAZONENSIS SB2B at 1.65 A resolution

Released:
DOI: 10.2210/pdb3mw8/pdb
PDB entry 3mw8 coloured by chain, front view.
PDB entry 3mw8 coloured by chain, side view.
PDB entry 3mw8 coloured by chain, top view.
Source organism: Shewanella amazonensis SB2B
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uroporphyrinogen-III synthase Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.75 KDa
Source organism: Shewanella amazonensis SB2B
Expression system: Escherichia coli
UniProt:
  • Canonical: A1SAQ1 (Residues: 1-239; Coverage: 100%)
Gene name: Sama_3255
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand EDO 12 x EDO
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 51.39Å b: 50.073Å c: 58.255Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.177 0.175 0.203
Expression system: Escherichia coli

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