› 3mwj

X-ray diffraction
1.4Å resolution

Q28E mutant of HERA N-terminal RecA-like domain, apo form

Released:
DOI: 10.2210/pdb3mwj/pdb
Model geometry
Fit model/data
PDB entry 3mwj coloured by chain, front view.
PDB entry 3mwj coloured by chain, side view.
PDB entry 3mwj coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Changing nucleotide specificity of the DEAD-box helicase Hera abrogates communication between the Q-motif and the P-loop.
Strohmeier J, Hertel I, Diederichsen U, Rudolph MG, Klostermeier D
Biol Chem 392 357-69 (2011)
PMID: 21391900

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.34 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 8-214; Coverage: 40%)
Gene name: TT_C1895
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 91.541Å b: 91.541Å c: 108.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.176
Expression system: Escherichia coli

Quick links

Citations

4 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
3 more

1 mention without citation

Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.
Chou et al. (2016)