› 3mx1

X-ray diffraction
2.3Å resolution

The structure of GIY-YIG endonuclease R.Eco29kI

Released:
DOI: 10.2210/pdb3mx1/pdb
PDB entry 3mx1 coloured by chain, front view.
PDB entry 3mx1 coloured by chain, side view.
PDB entry 3mx1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI.
Mak AN, Lambert AR, Stoddard BL
Structure 18 1321-31 (2010)
PMID: 20800503

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eco29kIR Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46944 (Residues: 2-214; Coverage: 100%)
Gene name: eco29kIR
Sequence domains: Eco29kI restriction endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1, ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 87.906Å b: 90.074Å c: 88.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.228
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.
Stoddard BL. (2011)
5 more

3 mentions without citation

Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI.
Mak et al. (2010)
2 more