Function and Biology Details
Reaction catalysed:
(1a) dTDP-beta-L-evernosamine + NADPH + O(2) = dTDP-N-hydroxy-beta-L-evernosamine + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Acyl-CoA dehydrogenase, C-terminal domain
- Acyl-CoA dehydrogenase-like, C-terminal
- Acyl-CoA oxidase/dehydrogenase, middle domain
- Acyl-CoA oxidase/dehydrogenase, middle domain superfamily
- Acyl-CoA dehydrogenase/oxidase, N-terminal
- Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily
- Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily
Structure analysis Details
Assembly composition:
homo tetramer (preferred)
Assembly name:
L-evernosamine nitrososynthase (preferred)
PDBe Complex ID:
PDB-CPX-236441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-evernosamine nitrososynthase
Molecule details ›
Chains: A, B, C, D
Length: 395 amino acids
Theoretical weight: 41.63 KDa
Source organism: Micromonospora sp. ATCC 39149
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 395 amino acids
Theoretical weight: 41.63 KDa
Source organism: Micromonospora sp. ATCC 39149
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
C4RPA1 (Residues: 10-404; Coverage: 94%)
Sequence domains:
- Acyl-CoA dehydrogenase, N-terminal domain
- Acyl-CoA dehydrogenase, middle domain
- Acyl-CoA dehydrogenase, C-terminal domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 21-ID-D
Spacegroup:
P65
Expression system: Escherichia coli BL21(DE3)
