› 3mya

X-ray diffraction
2.5Å resolution

Crystal Structure of HP67 H41F - P61

Released:
DOI: 10.2210/pdb3mya/pdb
PDB entry 3mya coloured by chain, front view.
PDB entry 3mya coloured by chain, side view.
PDB entry 3mya coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
On unsatisfied hydrogen bonds in the N-terminal subdomain of villin headpiece.
Brown JW, Farelli JD, McKnight CJ
J Mol Biol 413 543-7 (2011)
PMID: 21903098

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Villin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.62 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02640 (Residues: 760-826; Coverage: 8%)
Gene names: VIL, VIL1
Sequence domains: Villin headpiece domain
Structure domains: Villin headpiece domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P61
Unit cell:
a: 65.409Å b: 65.409Å c: 68.283Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

X-ray crystallographic validation of structure predictions used in computational design for protein stabilization.
Floor et al. (2015)
1 more

1 mention without citation

On unsatisfied hydrogen bonds in the N-terminal subdomain of villin headpiece.
Brown et al. (2011)