› 3mzh

X-ray diffraction
2.9Å resolution

Crystal structure of cAMP receptor protein from mycobacterium tuberculosis in complex with cAMP and its DNA binding element

Released:
DOI: 10.2210/pdb3mzh/pdb
PDB entry 3mzh coloured by chain, front view.
PDB entry 3mzh coloured by chain, side view.
PDB entry 3mzh coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Akhter Y, Wilmanns M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116598 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CRP-like cAMP-activated global transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.96 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMH3 (Residues: 1-224; Coverage: 100%)
Gene names: Rv3676, crp
Sequence domains:
Structure domains:
5'-D(P*AP*AP*AP*TP*GP*TP*GP*AP*TP*CP*TP*AP*GP*GP*TP*CP*AP*CP*GP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
5'-D(P*CP*AP*CP*GP*TP*GP*AP*CP*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.06 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 3 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 66.13Å b: 61.25Å c: 89.94Å
α: 90° β: 104.29° γ: 90°
R-values:
R R work R free
0.248 0.247 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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