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› 3mzy

X-ray diffraction
2.5Å resolution

The Crystal Structure of the RNA polymerase sigma-H factor from Fusobacterium nucleatum to 2.5A

Released:
DOI: 10.2210/pdb3mzy/pdb
Model geometry
Fit model/data
PDB entry 3mzy coloured by chain, front view.
PDB entry 3mzy coloured by chain, side view.
PDB entry 3mzy coloured by chain, top view.
Source organism: Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Entry authors: Stein AJ, Chhor G, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase sigma factor SigS Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 19.36 KDa
Source organism: Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8RIQ2 (Residues: 41-204; Coverage: 80%)
Gene name: FN1518
Sequence domains: Sigma-70 region 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 71.761Å b: 71.761Å c: 153.623Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.269
Expression system: Escherichia coli BL21(DE3)

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