› 3n4v

X-ray diffraction
2.4Å resolution

apo APH(2")-IVa form III

Released:
DOI: 10.2210/pdb3n4v/pdb
PDB entry 3n4v coloured by chain, front view.
PDB entry 3n4v coloured by chain, side view.
PDB entry 3n4v coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Crystal structure and kinetic mechanism of aminoglycoside phosphotransferase-2''-IVa.
Toth M, Frase H, Antunes NT, Smith CA, Vakulenko SB
Protein Sci 19 1565-76 (2010)
PMID: 20556826

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 35.33 KDa
Source organism: Enterococcus casseliflavus
Expression system: Not provided
UniProt:
  • Canonical: O68183 (Residues: 1-301; Coverage: 100%)
Gene name: aph(2'')-Id
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 75.966Å b: 65.119Å c: 78.458Å
α: 90° β: 91.68° γ: 90°
R-values:
R R work R free
0.196 0.193 0.263
Expression system: Not provided

Quick links

Citations

3 review citations

Prospects for circumventing aminoglycoside kinase mediated antibiotic resistance.
Shi et al. (2013)
2 more

2 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
1 more