› 3n53

X-ray diffraction
2.2Å resolution

Crystal structure of a response regulator receiver modulated diguanylate cyclase from Pelobacter carbinolicus

Released:
DOI: 10.2210/pdb3n53/pdb
PDB entry 3n53 coloured by chain, front view.
PDB entry 3n53 coloured by chain, side view.
PDB entry 3n53 coloured by chain, top view.
Source organism: Syntrophotalea carbinolica DSM 2380
Entry authors: Palani K, Zhang Z, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-174493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response receiver sensor diguanylate cyclase, GAF domain-containing Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 16.5 KDa
Source organism: Syntrophotalea carbinolica DSM 2380
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3A6W4 (Residues: 2-130; Coverage: 27%)
Gene name: Pcar_0634
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 34.004Å b: 44.932Å c: 88.681Å
α: 90° β: 94.37° γ: 90°
R-values:
R R work R free
0.269 0.252 0.286
Expression system: Escherichia coli BL21(DE3)

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