› 3n5a

X-ray diffraction
1.44Å resolution

Synaptotagmin-7, C2B-domain, calcium bound

Released:
DOI: 10.2210/pdb3n5a/pdb
PDB entry 3n5a coloured by chain, front view.
PDB entry 3n5a coloured by chain, side view.
PDB entry 3n5a coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7.
Xue M, Craig TK, Shin OH, Li L, Brautigam CA, Tomchick DR, Südhof TC, Rosenmund C, Rizo J
PLoS One 5 (2010)
PMID: 20824061

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-7 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9R0N7 (Residues: 266-403; Coverage: 34%)
Gene name: Syt7
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 35.598Å b: 44.892Å c: 87.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Distinct initial SNARE configurations underlying the diversity of exocytosis.
Kasai et al. (2012)
2 more

7 mentions without citation

The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.
MacDougall et al. (2018)
6 more