› 3n7o

X-ray diffraction
1.8Å resolution

X-ray structure of human chymase in complex with small molecule inhibitor.

Released:
DOI: 10.2210/pdb3n7o/pdb
PDB entry 3n7o coloured by chain, front view.
PDB entry 3n7o coloured by chain, side view.
PDB entry 3n7o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Potency variation of small-molecule chymase inhibitors across species.
Kervinen J, Crysler C, Bayoumy S, Abad MC, Spurlino J, Deckman I, Greco MN, Maryanoff BE, de Garavilla L
Biochem Pharmacol 80 1033-41 (2010)
PMID: 20599788

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Phe-|- > Tyr-|- > Trp-|- > Leu-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chymase Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.01 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23946 (Residues: 22-247; Coverage: 99%)
Gene names: CMA1, CYH, CYM
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand N7O 1 x N7O
Ligand UNL 2 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: I23
Unit cell:
a: 124.475Å b: 124.475Å c: 124.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.21
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Discovery and resupply of pharmacologically active plant-derived natural products: A review.
Atanasov et al. (2015)
3 more

5 mentions without citation

Granule Associated Serine Proteases of Hematopoietic Cells - An Analysis of Their Appearance and Diversification during Vertebrate Evolution.
Akula et al. (2015)
4 more