› 3n8i

X-ray diffraction
1.5Å resolution

Crystal structure of the A isoform of human cytoplasmic protein tyrosine phosphatase (HCPTP-A) in complex with 1-naphtylacetic acid

Released:
DOI: 10.2210/pdb3n8i/pdb
PDB entry 3n8i coloured by chain, front view.
PDB entry 3n8i coloured by chain, side view.
PDB entry 3n8i coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Homan KT, Balasubramaniam D, Stauffacher CV

Function and Biology Details

Reactions catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight phosphotyrosine protein phosphatase Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24666 (Residues: 2-158; Coverage: 99%)
Gene name: ACP1
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand NLA 1 x NLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 32.46Å b: 56.09Å c: 95.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.203
Expression system: Escherichia coli BL21(DE3)

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