› 3n9i

X-ray diffraction
1.95Å resolution

Crystal structure of tryptophanyl-tRNA synthetase from Yersinia pestis CO92

Released:
DOI: 10.2210/pdb3n9i/pdb
PDB entry 3n9i coloured by chain, front view.
PDB entry 3n9i coloured by chain, side view.
PDB entry 3n9i coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Nocek B, Maltseva N, Papazisi L, Anderson W, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 38.71 KDa
Source organism: Yersinia pestis CO92
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q8ZJF2 (Residues: 1-342; Coverage: 100%)
Gene names: YPO0157, YP_0159, trpS, y3940
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 61.099Å b: 78.15Å c: 152.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.167 0.206
Expression system: Cricetulus griseus

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