› 3n9k

X-ray diffraction
1.7Å resolution

F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A

Released:
DOI: 10.2210/pdb3n9k/pdb
PDB entry 3n9k coloured by chain, front view.
PDB entry 3n9k coloured by chain, side view.
PDB entry 3n9k coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
Carbohydrate binding sites in Candida albicans exo-β-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance.
Patrick WM, Nakatani Y, Cutfield SM, Sharpe ML, Ramsay RJ, Cutfield JF
FEBS J 277 4549-61 (2010)
PMID: 20875088

Function and Biology Details

Reaction catalysed: 
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucan 1,3-beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.62 KDa
Source organism: Candida albicans
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29717 (Residues: 40-438; Coverage: 96%)
Gene names: CAALFM_C102990CA, Ca49C10.05, CaO19.10507, CaO19.2990, EXG, EXG1, XOG, XOG1
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 58.725Å b: 64.397Å c: 94.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.197
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Occurrence and functional significance of secondary carbohydrate binding sites in glycoside hydrolases.
Cuyvers et al. (2012)
9 other articles

4 mentions without citation

Crystal structures of the laminarinase catalytic domain from Thermotoga maritima MSB8 in complex with inhibitors: essential residues for β-1,3- and β-1,4-glucan selection.
Jeng et al. (2011)
3 more