› 3nai

X-ray diffraction
2.56Å resolution

Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase

Released:
DOI: 10.2210/pdb3nai/pdb
PDB entry 3nai coloured by chain, front view.
PDB entry 3nai coloured by chain, side view.
PDB entry 3nai coloured by chain, top view.
Source organism: Murine norovirus 1
Entry authors: Kim KH, Lee JH, Alam I, Park Y, Kang S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 517 amino acids
Theoretical weight: 58.52 KDa
Source organism: Murine norovirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80J95 (Residues: 1181-1686; Coverage: 30%)
Gene name: ORF1
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 3 x MG
Ligand URF 3 x URF
Ligand MN3 6 x MN3
Ligand SO4 14 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: C2
Unit cell:
a: 121.246Å b: 196.562Å c: 109.598Å
α: 90° β: 114.81° γ: 90°
R-values:
R R work R free
0.206 0.203 0.268
Expression system: Escherichia coli

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