› 3nal

X-ray diffraction
2.65Å resolution

SR Ca(2+)-ATPase in the HnE2 state complexed with the Thapsigargin derivative DTB

Released:
DOI: 10.2210/pdb3nal/pdb
PDB entry 3nal coloured by chain, front view.
PDB entry 3nal coloured by chain, side view.
PDB entry 3nal coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Critical roles of hydrophobicity and orientation of side chains for inactivation of sarcoplasmic reticulum Ca2+-ATPase with thapsigargin and thapsigargin analogs.
Winther AM, Liu H, Sonntag Y, Olesen C, le Maire M, Soehoel H, Olsen CE, Christensen SB, Nissen P, Møller JV
J Biol Chem 285 28883-92 (2010)
PMID: 20551329

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand MG 1 x MG
Ligand DBK 1 x DBK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 71.33Å b: 71.33Å c: 591.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.283

Quick links

Citations

10 review citations

Endoplasmic reticulum stress: its role in disease and novel prospects for therapy.
Schönthal AH. (2012)
9 more

7 mentions without citation

Ion pathways in the sarcoplasmic reticulum Ca2+-ATPase.
Bublitz et al. (2013)
6 more