› 3nbu

X-ray diffraction
2.05Å resolution

Crystal structure of pGI glucosephosphate isomerase

Released:
DOI: 10.2210/pdb3nbu/pdb
PDB entry 3nbu coloured by chain, front view.
PDB entry 3nbu coloured by chain, side view.
PDB entry 3nbu coloured by chain, top view.
Source organism: Escherichia coli DH5[alpha]
Primary publication:
Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization.
Totir M, Echols N, Nanao M, Gee CL, Moskaleva A, Gradia S, Iavarone AT, Berger JM, May AP, Zubieta C, Alber T
PLoS One 7 e32498 (2012)
PMID: 22393408

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 549 amino acids
Theoretical weight: 61.63 KDa
Source organism: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P0A6T1 (Residues: 1-549; Coverage: 100%)
Gene names: JW3985, b4025, pgi
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 69.806Å b: 72.874Å c: 181.851Å
α: 92.47° β: 97.82° γ: 114.57°
R-values:
R R work R free
0.172 0.169 0.23

Quick links

Citations

4 review citations

Current trends in protein crystallization.
Gavira JA. (2016)
3 more

3 mentions without citation

Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization.
Totir et al. (2012)
2 more