› 3nch

X-ray diffraction
2.88Å resolution

Yeast Glycogen Synthase (Gsy2p) Basal State Conformation

Released:
DOI: 10.2210/pdb3nch/pdb
PDB entry 3nch coloured by chain, front view.
PDB entry 3nch coloured by chain, side view.
PDB entry 3nch coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran S, Roach PJ, DePaoli-Roach AA, Hurley TD
Proc Natl Acad Sci U S A 107 17563-8 (2010)
PMID: 20876143

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 725 amino acids
Theoretical weight: 82.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27472 (Residues: 1-705; Coverage: 100%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand SO4 15 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 93.94Å b: 161.837Å c: 121.622Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.215 0.213 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Glycogen and its metabolism: some new developments and old themes.
Roach et al. (2012)
10 more

2 mentions without citation

Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran et al. (2010)
1 more