› 3ncx

X-ray diffraction
2.6Å resolution

Crystal structure of EHEC O157:H7 intimin mutant

Released:
DOI: 10.2210/pdb3ncx/pdb
PDB entry 3ncx coloured by chain, front view.
PDB entry 3ncx coloured by chain, side view.
PDB entry 3ncx coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. TW14359
Primary publication:
Crystal structure of EHEC intimin: insights into the complementarity between EPEC and EHEC.
Yi Y, Ma Y, Gao F, Mao X, Peng H, Feng Y, Fan Z, Wang G, Guo G, Yan J, Zeng H, Zou Q, Gao GF
PLoS One 5 e15285 (2010)
PMID: 21179574

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intimin Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 20.72 KDa
Source organism: Escherichia coli O157:H7 str. TW14359
Expression system: Escherichia coli
UniProt:
  • Canonical: P43261 (Residues: 747-934; Coverage: 20%)
Gene names: ECs4559, L0025, Z5110, eae, eaeA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 43.771Å b: 92.488Å c: 100.048Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.268
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Immunoglobulin domains in Escherichia coli and other enterobacteria: from pathogenesis to applications in antibody technologies.
Bodelón et al. (2013)
11 other articles

1 mention without citation

Crystal structure of EHEC intimin: insights into the complementarity between EPEC and EHEC.
Yi et al. (2010)