› 3nd7

X-ray diffraction
2.4Å resolution

Crystal structure of phosphopantetheine adenylyltransferase from Enterococcus faecalis in the ligand-unbound state and in complex with ATP and pantetheine

Released:
DOI: 10.2210/pdb3nd7/pdb
PDB entry 3nd7 coloured by chain, front view.
PDB entry 3nd7 coloured by chain, side view.
PDB entry 3nd7 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Crystal structure of phosphopantetheine adenylyltransferase from Enterococcus faecalis in the ligand-unbound state and in complex with ATP and pantetheine.
Yoon HJ, Kang JY, Mikami B, Lee HH, Suh SW
Mol Cells 32 431-5 (2011)
PMID: 21912874

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 171 amino acids
Theoretical weight: 19.77 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q831P9 (Residues: 1-163; Coverage: 100%)
Gene names: EF_2451, coaD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand PNY 6 x PNY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 109.64Å b: 125.791Å c: 125.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural Biology and the Design of New Therapeutics: From HIV and Cancer to Mycobacterial Infections: A Paper Dedicated to John Kendrew.
Thomas et al. (2017)
4 other articles

2 mentions without citation

Discovery of inhibitors of 4'-phosphopantetheine adenylyltransferase (PPAT) to validate PPAT as a target for antibacterial therapy.
de Jonge et al. (2013)
1 more