› 3ngh

X-ray diffraction
1.8Å resolution

Molecular Analysis of the Interaction of the HDL Receptor SR-BI with the Adaptor Protein PDZK1

Released:
DOI: 10.2210/pdb3ngh/pdb
PDB entry 3ngh coloured by chain, front view.
PDB entry 3ngh coloured by chain, side view.
PDB entry 3ngh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
In vitro and in vivo analysis of the binding of the C terminus of the HDL receptor scavenger receptor class B, type I (SR-BI), to the PDZ1 domain of its adaptor protein PDZK1.
Kocher O, Birrane G, Tsukamoto K, Fenske S, Yesilaltay A, Pal R, Daniels K, Ladias JA, Krieger M
J Biol Chem 285 34999-5010 (2010)
PMID: 20739281

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JIL4 (Residues: 7-110; Coverage: 20%)
Gene names: Cap70, Nherf3, Pdzk1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 35.604Å b: 55.114Å c: 111.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.238
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Regulation of G protein-coupled receptor function by Na+/H+ exchange regulatory factors.
Ardura et al. (2011)
8 more

3 mentions without citation

Stereochemical determinants of C-terminal specificity in PDZ peptide-binding domains: a novel contribution of the carboxylate-binding loop.
Amacher et al. (2013)
2 more